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Yatalase(Fungal cell wall lytic enzyme)
It is possible to prepare protoplasts from filamentous fungi and easily measure the amount of koji fungus.
It is possible to lyse the cell walls of filamentous fungi and prepare intracellular enzymes.
| Model | Sales destination | Sales Code No. | Eur-lex.europa.eu eur-lex.europa.eu |
| Yatalase | Takara Bio Inc. | T017 | 2 g/vial |
| Yatalase | Cosmo Bio Co., Ltd. | OZ-10EX | 2 g/vial |
| Features | It can powerfully decompose raw chitin and has chitinase and chitobiase activity. It can be used alone to prepare protoplasts of filamentous fungi. |
| Shape | Lyophilized powder (contains lactose as an excipient) |
| save | 4℃, dry conditions |
| 起源 | Corynebacterium sp. OZ-21 |
| Specification | Chitinase activity 50 units/g powder or more, chitobiase activity 500 units/g powder or more, Cell wall lytic activity approximately 10,000 units/g powder |
| 至適温度 | 30 ~ 50 |
| Optimal pH | 5 to 8 |
application
A simple method for measuring koji fungus mass using Yatalase
| Features | No enzyme preparation is required, and Yatalase alone can measure bacterial mass. The procedure is simple, and the reaction time is short, allowing measurements to be made within 4 hours. |
| Operating Procedure |  |
| Measurement result example |   |
literature
- Reissig, J. L., Strominger, J. L. and Leloir, L. F. (1955).
J. Biol. Chem. 217, 959. - Fujii, F., Ozeki, K., Kanda, A., Hamaji, M., and Nunokawa, Y. (1992).
Brewers Association 87, 757. - Ozeki, K., Ishimaru (Nagamasu), Y., Koda, A., Minetoki, T., Obuchi, K., Hamaji, M., and Kumagai, C. (1998).
bioengineering 76, 187
FAQ
- Is it effective against any type of filamentous fungus?
- Aspergillus oryzaeとTrichoderma reesei,T.viride,T. koningiican be used to prepare protoplasts very efficiently and in reduced amounts.
OtherAspergillusIf protoplasts are difficult to obtain from Ascomycetes, Zygomycetes, and Basidiomycetes, including those of the genus Pseudomonas, we recommend using it in combination with 0.5% cellulase "Onozuka" R-10.