Yatalase-Plus-(Fungal cell wall lytic enzyme)
Protoplasts can also be efficiently prepared from filamentous fungi such as black koji mold.
| Model | Sales destination | Sales Code No. | Eur-lex.europa.eu eur-lex.europa.eu |
| Yatalase -Plus- |
Takara Bio Inc. | T030 | 2 gr |
| Features | It is a complex enzyme preparation with chitinase, chitobiase, and α-1,3-glucanase activities. It can prepare protoplasts of filamentous fungi by itself, and can efficiently produce protoplasts of filamentous fungi such as black koji mold, which are difficult to produce protoplasts with Yatalase. |
| Shape | Lyophilized powder (contains lactose as an excipient) |
| save | 4℃, dry conditions |
| Specification | Chitinase activity 50 U/g powder or more, chitobiase activity 500 U/g powder or more, α-1,3-glucanase activity: 20 U/g powder or more |
| 至適温度 | 30 ℃ |
| Optimal pH | 6.0 |
| Properties |
|
application
VariousAspergillusProtoplast formation for genus
Example of protoplast preparation conditions
- (1)Aspergillus niger, Aspergillus luchuensis, Aspergillus sojae, Aspergillus nidulans, Aspergillus kawachii
- Culture medium: Dextrin-peptone, 200 mM sodium phosphate buffer (pH 7.0)
- Culture conditions: 30℃, 42 hr., Shake culture (Rotary at 140 rpm)
- Preparation conditions: 2% Yatalase-Plus- solution, 0.8 M NaCl, 50 mM sodium phosphate buffer (pH 6.0)
30℃ Reciprocal shaking (at 60 to 70 rpm) 3 hr.
- (2)Aspergillus oryzae, Aspergillus aculeatus
- Dextrin-peptone medium
- Culture conditions, preparation conditions: Same as above