Expression system characteristics

High secretory productivity

Expression success rate: 86%

Glycosylation

Safe & secure

Industrial enzymes and food processing enzymes
We will build a mass production system.

Koji mold (Aspergillus oryzae) has been used for hundreds of years to produce fermented foods such as sake, soy sauce, and miso, and is recognized for its high safety.
In addition, due to its high protein production capacity, it is attracting attention as a host for protein production.

We have successfully constructed a protein expression system using koji mold and are now offering contract protein expression services.
There are many examples of successful mass production.

Expression results

Origin

Proteins derived from filamentous fungi

The success rate of expression of proteins derived from filamentous fungi is extremely high, with productivity of up to 10 g/L or more being achieved.

◎Examples of manifestation

foreign proteins

Among heterologous proteins, microbial-derived proteins show relatively high productivity.

◎Examples of manifestation

Contract track record

production amount

Flow of contracted services

Basic Work

Expression vector construction (~2 weeks)

The target gene is inserted into our high-expression vector, a transformation vector is constructed, and the sequence is then confirmed.

Obtaining and purifying transformants (up to 4 weeks)

The koji mold is transformed, and after screening for transformants, they are purified by subculture.

Confirmation of expression (up to 4 weeks)

Using the obtained transformants, expression is confirmed by SDS-PAGE or Western analysis, and the number of expression cassettes introduced is estimated by real-time PCR.

Preparation of offerings (~2 weeks)

The transformants are cultured, and after harvesting, we provide the culture supernatant or bacterial cell extract.

Delivery

Approximately 3 months from gene provision (+1 month for total gene synthesis)

Provided (results)

• Culture supernatant...approx. 20 mL
  (Or, approximately 2 mL of bacterial extract)
  *The amount can be increased upon request.
  (A separate quote may be required).
• 業務報告書

Option

Codon modification

The gene is designed and totally synthesized to match the codon usage frequency of koji mold.

Scale-up culture

The transformants are cultured in a jar fermenter and the culture supernatant is provided.

Obtaining multicopy strains

We will expand the screening scale and select high-copy strains.

How to place an order

Your order will be kept strictly confidential.
We will enter into a confidentiality agreement as necessary.
We will provide you with a quote for price and delivery time each time.
Please feel free to contact us for details.

• All services do not guarantee expression or activity.

References

• Development of a mass protein production system using Aspergillus oryzae
  Akio Koda, Hirokazu Tsuboi, Toshiki Minetoki, and Takayuki Bogaki
  Protein Science Society Archives, 9, e084 (2016)
• AspergillusHeterologous gene expression systems of the genus
  Toshiki Minetoki, Chemistry and Biology, 38, 831-838 (2000).
• Improving the efficiency of heterologous protein production by improving post-transcriptional processes
  Akio Koda, Masafumi Tokuoka, Katsuya Gomi, Bioscience and Industry,
  63, 543-546 (2005).
• With the introduction of the cis elements Aspergillus oryzae Improvement of the enolase promoter
  Tsuboi, H. et al., Biosci. Biotechnol. Biochem.,
  69, 206-208 (2005)